ORF8, SARS-CoV-2, COVID-19, Truncated, Intrinsically disordered region, Truncation mutation, Continent distribution
Digital Object Identifier (DOI)
Open reading frame 8 (ORF8) shows one of the highest levels of variability among accessory proteins in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus Disease 2019 (COVID-19). It was previously reported that the ORF8 protein inhibits the presentation of viral antigens by the major histocompatibility complex class I (MHC-I), which interacts with host factors involved in pulmonary inflammation. The ORF8 protein assists SARS-CoV-2 in evading immunity and plays a role in SARS-CoV-2 replication. Among many contributing mutations, Q27STOP, a mutation in the ORF8 protein, defines the B.1.1.7 lineage of SARS-CoV-2, engendering the second wave of COVID-19. In the present study, 47 unique truncated ORF8 proteins (T-ORF8) with the Q27STOP mutations were identified among 49,055 available B.1.1.7 SARS-CoV-2 sequences. The results show that only one of the 47 T-ORF8 variants spread to over 57 geo-locations in North America, and other continents, which include Africa, Asia, Europe and South America. Based on various quantitative features, such as amino acid homology, polar/non-polar sequence homology, Shannon entropy conservation, and other physicochemical properties of all specific 47 T-ORF8 protein variants, nine possible T-ORF8 unique variants were defined. The question as to whether T-ORF8 variants function similarly to the wild type ORF8 is yet to be investigated. A positive response to the question could exacerbate future COVID-19 waves, necessitating severe containment measures.
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Citation / Publisher Attribution
PeerJ, v. 10, art. e13136
Scholar Commons Citation
Hassan, Sk. Sarif; Kodakandla, Vaishnavi; Redwan, Elrashdy M.; Lundstrom, Kenneth; Choudhury, Pabitra Pal; El-Aziz, Tarek Mohamed; Takayama, Kazuo; Kandimalla, Ramesh; Lal, Amos; Serrano-Aroca, Ángel; Azad, Gajendra Kumar; Aljabali, Alaa A.A.; Palù, Giorgio; Chauhan, Gaurav; Adadi, Parise; Tambuwala, Murtaza; Brufsky, Adam M.; Baetas-da-Cruz, Wagner; Barh, Debmalya; Azevedo, Vasco; Bazan, Nikolas G.; Andrade, Bruno Silva; Silva, Raner José; and Uversky, Vladimir N., "An Issue of Concern: Unique Truncated ORF8 Protein Variants of SARS-CoV-2" (2022). Molecular Medicine Faculty Publications. 924.