Cytokine, Interferon, S100 Protein, Protein–protein Interaction, Cancer
Digital Object Identifier (DOI)
Interferon-β (IFN-β) is a pleiotropic cytokine used for therapy of multiple sclerosis, which is also effective in suppression of viral and bacterial infections and cancer. Recently, we reported a highly specific interaction between IFN-β and S100P lowering IFN-β cytotoxicity to cancer cells (Int J Biol Macromol. 2020; 143: 633–639). S100P is a member of large family of multifunctional Ca2+-binding proteins with cytokine-like activities. To probe selectivity of IFN-β—S100 interaction with respect to S100 proteins, we used surface plasmon resonance spectroscopy, chemical crosslinking, and crystal violet assay. Among the thirteen S100 proteins studied S100A1, S100A4, and S100A6 proteins exhibit strictly Ca2+-dependent binding to IFN-β with equilibrium dissociation constants, Kd, of 0.04–1.5 µM for their Ca2+-bound homodimeric forms. Calcium depletion abolishes the S100—IFN-β interactions. Monomerization of S100A1/A4/A6 decreases Kd values down to 0.11–1.0 nM. Interferon-α is unable of binding to the S100 proteins studied. S100A1/A4 proteins inhibit IFN-β-induced suppression of MCF-7 cells viability. The revealed direct influence of specific S100 proteins on IFN-β activity uncovers a novel regulatory role of particular S100 proteins, and opens up novel approaches to enhancement of therapeutic efficacy of IFN-β.
This work is licensed under a Creative Commons Attribution 4.0 License.
Was this content written or created while at USF?
Citation / Publisher Attribution
International Journal of Molecular Sciences, v. 21, issue 24, art. 9473
Scholar Commons Citation
Kazakov, Alexei S.; Sofin, Alexander D.; Avkhacheva, Nadezhda V.; Denesyuk, Alexander I.; Deryusheva, Eugenia I.; Rastrygina, Victoria A.; Sokolov, Andrey S.; Permyakova, Maria E.; Litus, Ekaterina A.; Uversky, Vladimir N.; Permyakov, Eugene A.; and Permyakov, Sergei E., "Interferon Beta Activity Is Modulated Via Binding of Specific S100 Proteins" (2020). Molecular Medicine Faculty Publications. 821.