Coupled Folding and Binding with α-helix-forming Molecular Recognition Elements
Algorithms, Cell Signaling, Diseases and Disorders, Genomics, Peptides and Proteins
Digital Object Identifier (DOI)
Many protein−protein and protein−nucleic acid interactions involve coupled folding and binding of at least one of the partners. Here, we propose a protein structural element or feature that mediates the binding events of initially disordered regions. This element consists of a short region that undergoes coupled binding and folding within a longer region of disorder. We call these features “molecular recognition elements” (MoREs). Examples of MoREs bound to their partners can be found in the α-helix, β-strand, polyproline II helix, or irregular secondary structure conformations, and in various mixtures of the four structural forms. Here we describe an algorithm that identifies regions having propensities to become α-helix-forming molecular recognition elements (α-MoREs) based on a discriminant function that indicates such regions while giving a low false-positive error rate on a large collection of structured proteins. Application of this algorithm to databases of genomics and functionally annotated proteins indicates that α-MoREs are likely to play important roles protein−protein interactions involved in signaling events.
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Citation / Publisher Attribution
Biochemistry, v. 44, issue 37, p. 12454-12470
Scholar Commons Citation
Oldfield, Christopher J.; Cheng, Yugong; Cortese, Marc S.; Romero, Pedro R.; Uversky, Vladimir N.; and Dunker, A. Keith, "Coupled Folding and Binding with α-helix-forming Molecular Recognition Elements" (2005). Molecular Medicine Faculty Publications. 747.