How to Improve Nature: Study of The Electrostatic Properties of The Surface of α-lactalbumin
Digital Object Identifier (DOI)
It was recently shown that α-lactalbumin interacts with histones and simple models of histone proteins such as positively charged polyamino acids, suggesting that some fundamental aspects of the protein surface electrostatics may come into play. In the present work, the energies of charge–charge interaction in apo- and Ca2+-loaded α-lactalbumin were calculated using a Tanford–Kirkwood algorithm with either solvent accessibility correction or using a finite difference Poisson–Boltzmann method. The analysis revealed two major regions of α-lactalbumin that possessed highly unfavorable electrostatic potentials: (a) the Ca2+-binding loop and its neighboring residues and (b) the N-terminal region of the protein. Several individual mutants were prepared to neutralize specific individual surface acidic amino acids at both the N-terminus and Ca2+-binding loop of bovine α-lactalbumin. These mutants were characterized by intrinsic fluorescence, differential scanning microcalorimetry and circular dichroism. The structural and thermodynamic data agree in every case with the theoretical predictions, confirming that the N-terminal region is very sensitive to changes in charge. For example, desMet D14N mutation destabilizes protein and decreases its calcium affinity. On the other hand, desMet E1M and desMet D37N substitutions increase the thermal stability and calcium affinity. The Met E1Q is characterized by a marked increase in protein stability, whereas desMet E7Q and desMet E11L display a slight increase in calcium affinity and thermal stability. Examination of the unfavorable energy contributed by Glu1 and the energetically favorable consequences of neutralizing this residue suggests that nature may have made an error with bovine α-lactalbumin from the viewpoint of stabilizing structure and conformation.
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Citation / Publisher Attribution
Protein Engineering, Design and Selection, v. 18, issue 9, p. 425-433
Scholar Commons Citation
Permyakov, Sergei E.; Makhatadze, George I.; Owenius, Rikard; Uversky, Vladimir N.; Brooks, Charles L.; Permyakov, Eugene A.; and Berliner, Lawrence J., "How to Improve Nature: Study of The Electrostatic Properties of The Surface of α-lactalbumin" (2005). Molecular Medicine Faculty Publications. 746.