Conversion of Human α-lactalbumin to an Apo-like State in The Complexes with Basic Poly-amino Acids: Toward Understanding of The Molecular Mechanism of Antitumor Action of HAMLET
R-lactalbumin, Histones, Poly-amino Acids, Electrostatics, Thermal Stability, Calcium-binding
Digital Object Identifier (DOI)
It was recently shown that α-lactalbumin associated with oleic acid (HAMLET) interacts with core histones thereby triggering apoptosis of tumor cells (J. Biol. Chem.2003, 278, 42131). In previous work, we revealed that monomeric α-lactalbumin in the absence of fatty acids can also interact with histones and, moreover, with basic poly-amino acids (poly-Lys and poly-Arg) that represent simple models of histone proteins (Biochemistry2004, 43, 5575). Association of α-lactalbumin with histone or poly-Lys(Arg) essentially changes its properties. In the present work, the character of the changes in structural properties and conformational stability of α-lactalbumin in the complex with poly-Lys(Arg) has been studied in detail by steady-state fluorescence, circular dichroism, and differential scanning calorimetry. Complex formation strongly depends on ionic strength, confirming its electrostatic nature. Experiments with the poly-amino acids of various molecular masses demonstrated a direct proportionality between the number of α-lactalbumin molecules bound per poly-Lys(Arg) and the surface area of the poly-amino acid random coil. The binding of the poly-amino acids to Ca2+-saturated human α-lactalbumin decreases its thermal stability down to the level of its free apo-form and decreases Ca2+-affinity by 4 orders of magnitude. The conformational state of α-lactalbumin in a complex with poly-Lys(Arg), named α-LActalbumin Modified by Poly-Amino acid (LAMPA), differs from all other α-lactalbumin states characterized to date, representing an apo-like (molten globule-like) state with substantially decreased affinity for calcium ion. The requirement for efficient conversion of α-lactalbumin to the LAMPA state is a poly-Lys(Arg) chain consisting of several tens of amino acid residues.
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Citation / Publisher Attribution
Journal of Proteome Research, v. 4, issue 2, p. 211-640
Scholar Commons Citation
Permyakov, Sergei E.; Pershikova, Irina V.; Zhadan, Andrei P.; Goers, John; Bakunts, Anush G.; Uversky, Vladimir N.; Berliner, Lawrence J.; and Permyakov, Eugene A., "Conversion of Human α-lactalbumin to an Apo-like State in The Complexes with Basic Poly-amino Acids: Toward Understanding of The Molecular Mechanism of Antitumor Action of HAMLET" (2005). Molecular Medicine Faculty Publications. 739.