Actinous Enigma Or Enigmatic Actin: Folding, Structure, and Functions of the Most Abundant Eukaryotic Protein
Actin, Multifunctional Protein, Microfilaments, Signal Transduction, Hub Protein
Digital Object Identifier (DOI)
Being the most abundant protein of the eukaryotic cell, actin continues to keep its secrets for more than 60 years. Everything about this protein, its structure, functions, and folding, is mysteriously counterintuitive, and this review represents an attempt to solve some of the riddles and conundrums commonly found in the field of actin research. In fact, actin is a promiscuous binder with a wide spectrum of biological activities. It can exist in at least three structural forms, globular, fibrillar, and inactive (G-, F-, and I-actin, respectively). G-actin represents a thermodynamically instable, quasi-stationary state, which is formed in vivo as a result of the energy-intensive, complex posttranslational folding events controlled and driven by cellular folding machinery. The G-actin structure is dependent on the ATP and Mg2+ binding (which in vitro is typically substituted by Ca2+) and protein is easily converted to the I-actin by the removal of metal ions and by action of various denaturing agents (pH, temperature, and chemical denaturants). I-actin cannot be converted back to the G-form. Foldable and “natively folded” forms of actin are always involved in interactions either with the specific protein partners, such as Hsp70 chaperone, prefoldin, and the CCT chaperonin during the actin folding in vivo or with Mg2+ and ATP as it takes place in the G-form. We emphasize that the solutions for the mysteries of actin multifunctionality, multistructurality, and trapped unfolding can be found in the quasi-stationary nature of this enigmatic protein, which clearly possesses many features attributed to both globular and intrinsically disordered proteins.
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Citation / Publisher Attribution
Intrinsically Disordered Proteins, v. 2, issue 1, art. e34500
Scholar Commons Citation
Povarova, Olga I.; Uversky, Vladimir N.; Kuznetsova, Irina M.; and Turoverov, Konstantin K., "Actinous Enigma Or Enigmatic Actin: Folding, Structure, and Functions of the Most Abundant Eukaryotic Protein" (2014). Molecular Medicine Faculty Publications. 582.