Fluorescence, Amyloid Proteins, Viscosity, Glycerol, Fluorescent Dyes, Insulin, Solvents, Absorption Spectra
Digital Object Identifier (DOI)
In this work, the fluorescence of thioflavin T (ThT) was studied in a wide range of viscosity and temperature. It was shown that ThT fluorescence quantum yield varies from 0.0001 in water at room temperature to 0.28 in rigid isotropic solution (T/η→0). The deviation of the fluorescence quantum yield from unity in rigid isotropic solution suggests that fluorescence quantum yield depends not only on the ultra-fast oscillation of ThT fragments relative to each other in an excited state as was suggested earlier, but also depends on the molecular configuration in the ground state. This means that the fluorescence quantum yield of the dye incorporated into amyloid fibrils must depend on its conformation, which, in turn, depends on the ThT environment. Therefore, the fluorescence quantum yield of ThT incorporated into amyloid fibrils can differ from that in the rigid isotropic solution. In particular, the fluorescence quantum yield of ThT incorporated into insulin fibrils was determined to be 0.43. Consequently, the ThT fluorescence quantum yield could be used to characterize the peculiarities of the fibrillar structure, which opens some new possibilities in the ThT use for structural characterization of the amyloid fibrils.
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Citation / Publisher Attribution
PLoS ONE, v. 5, issue 10, art. e15385
© 2010 Sulatskaya, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Scholar Commons Citation
Sulatskaya, Anna I.; Maskevich, Alexander A.; Kuznetsova, Irina M.; Uversky, Vladimir N.; and Turoverov, Konstantin K., "Fluorescence Quantum Yield of Thioflavin T in Rigid Isotropic Solution and Incorporated into The Amyloid Fibrils" (2010). Molecular Medicine Faculty Publications. 455.