Document Type

Article

Publication Date

2015

Keywords

macromolecular crowding, excluded volume effect, viscosity, soft interactions, solvent properties, aqueous two-phase system, partition

Digital Object Identifier (DOI)

https://doi.org/10.3390/molecules20011377

Abstract

Macromolecular crowding is known to affect protein folding, binding of small molecules, interaction with nucleic acids, enzymatic activity, protein-protein interactions, and protein aggregation. Although for a long time it was believed that the major mechanism of the action of crowded environments on structure, folding, thermodynamics, and function of a protein can be described in terms of the excluded volume effects, it is getting clear now that other factors originating from the presence of high concentrations of “inert” macromolecules in crowded solution should definitely be taken into account to draw a more complete picture of a protein in a crowded milieu. This review shows that in addition to the excluded volume effects important players of the crowded environments are viscosity, perturbed diffusion, direct physical interactions between the crowding agents and proteins, soft interactions, and, most importantly, the effects of crowders on solvent properties.

Rights Information

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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Yes

Citation / Publisher Attribution

Molecules, v. 20, issue 1, p. 1377-1409

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