Spliceosome, Intrinsically disordered protein, Protein structure, RNA–protein complex, Protein–protein interaction, Intrinsic disorder, Protein–RNA interaction, Protein hub, Splicing, Protein function
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Recent studies revealed that a significant fraction of any given proteome is presented by proteins that do not have unique 3D structures as a whole or in significant parts. These intrinsically disordered proteins possess dramatic structural and functional variability, being especially enriched in signaling and regulatory functions since their lack of fixed structure defines their ability to be involved in interaction with several proteins and allows them to be re-used in multiple pathways. Among recognized disorder-based protein functions are interactions with nucleic acids and multi-target binding; i.e., the functions ascribed to many spliceosomal proteins. Therefore, the spliceosome, a multimegadalton ribonucleoprotein machine catalyzing the excision of introns from eukaryotic pre-mRNAs, represents an attractive target for the focused analysis of the abundance and functionality of intrinsic disorder in its proteinaceous components. In yeast cells, spliceosome consists of five small nuclear RNAs (U1, U2, U4, U5, and U6) and a range of associated proteins. Some of these proteins constitute cores of the corresponding snRNA-protein complexes known as small nuclear ribonucleoproteins (snRNPs). Other spliceosomal proteins have various auxiliary functions. To gain better understanding of the functional roles of intrinsic disorder, we have studied the prevalence of intrinsically disordered proteins in the yeast spliceosome using a wide array of bioinformatics methods. Our study revealed that similar to the proteins associated with human spliceosomes (Korneta & Bujnicki, 2012), proteins found in the yeast spliceosome are enriched in intrinsic disorder.
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Citation / Publisher Attribution
PeerJ, v. 1, art. e2
Scholar Commons Citation
Ribeiro, Maria de Lourdes Coelho; Espinosa, Julio; Islam, Sameen; Martinez, Osvaldo; Thanki, Jayesh Jamnadas; Mazariegos, Stephanie; Nguyen, Tam; Larina, Maya; Xue, Bin; and Uversky, Vladimir N., "Malleable Ribonucleoprotein Machine: Protein Intrinsic Disorder in the Saccharomyces cerevisiae Spliceosome" (2013). Molecular Medicine Faculty Publications. 2.