A procedure for enriching and cleaning up Rhodamine B and rhodamine WT in natural waters, using a Sep-pak C18 cartridge

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Publication Date

1-1-1984

Publication Title

Water Research

Volume Number

18

Issue Number

2

Abstract

An enrichment and clean-up procedure is described for Rhodamine B and Rhodamine WT in natural waters. Sep-pak C18 cartridges were used to adsorb the Rhodamine dyes from aqueous solutions. Complete desorption of Rhodamine B and Rhodamine WT on the Sep-pak was achieved. The concentration of the dyes could be increased more than a 100-fold. The concentration of Rhodamine B in the North Sea during a tracer experiment was measured using three different methods. The Sep-pak procedure gave lower concentrations of Rhodamine B than the direct fluorometric measurements. It is concluded that when the Sep-pak procedure is used to enrich Rhodamine dyes there is no interference from salts, pH, pigments and natural fluorescence on the dye fluorescence. This enrichment and clean-up procedure has advantages in tracer experiments. With the same amount of dye it is possible to measure for a longer period. For short experiments, less dye needs to be used.

Keywords

Fluorescent tracers, Fluorescence spectroscopy, Water quality monitoring, Chemical tests and reagents, Organic compounds

Document Type

Article

Digital Object Identifier (DOI)

https://doi.org/10.1016/0043-1354(84)90064-2

Language

English

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