Pyranine used as a fluorescent tracer in hydrology: pH effects in determination of its concentration

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Publication Date

4-1-1980

Publication Title

Journal of Hydrology

Volume Number

46

Issue Number

3-4

Abstract

Pyranine is used as a fluorescent tracer in hydrology. But, this compound which may exist under several ionic forms has, for a given concentration a fluorescence emission which depends strongly on the pH. We proposed a simple method for the measurement of fluorescence intensity of the tracer by buffering solutions at fixed pH values: pH 10 (excitation at 450 nm, emission at 515 nm) or pH 5 (excitation at 403 nm, emission at 515 nm). In both cases, the limit of detection is close to 4 · 10−10 g/g.

Keywords

Fluorescence spectroscopy, Fluorescent tracers, Hydrology, Tracers, Acid-base chemistry

Document Type

Article

Digital Object Identifier (DOI)

https://doi.org/10.1016/0022-1694(80)90088-8

Language

English

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