A cryopreservation protocol for embryos of the endangered species Zizania texana.
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Publication Date
January 2002
Abstract
Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.
Document Type
Article
Notes
Cryo letters, Vol. 23, no. 5 (2002).
Identifier
SFS0070074_00001
Recommended Citation
Walters, Christina; Touchell, Darren H.; and Power, Paula, "A cryopreservation protocol for embryos of the endangered species Zizania texana." (2002). KIP Articles. 1175.
https://digitalcommons.usf.edu/kip_articles/1175
