Thesis Director: David John, Ph.D. Professor, College of Arts and Sciences
University of South Florida St. Petersburg
It was hypothesized that rinsing a toothbrush in mouthwash after each use would reduce the amount of microbes that would grow on the toothbrush. Group 1 rinsed their toothbrushes in just water, and Group 2 rinsed their toothbrush in mouthwash after each use. Toothbrushes were collected and eluates were spread onto mannitol salt agars, eosin-methylene blue agars, blood agars, Sabouraud agars, and TYCSB agars and allowed to grow. Colony counts for all trials were converted to mean percent positives and mean percent positives >10. The difference in percent positives and percent positives >10 were statistically significant for p>0.1 for the mannitol salt, eosin-methylene blue, blood, and TYCSB agars. Overall, Group 2 had less microbial growth than Group 1 which supports the hypothesis. There was an unexplained, drastic variance in colony counts for the Sabouraud agars for both groups and therefore the results were not statistically significant.
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Carmichael, Lindsey, "Effectiveness of Mouthwash Rinsing in Limiting Microbial Growth on Toothbrushes" (2016). USF St. Petersburg campus Honors Program Theses (Undergraduate).