Graduation Year


Document Type




Degree Name

Master of Science (M.S.)

Degree Granting Department


Major Professor

Theresa Evans-Nguyen, Ph.D.

Committee Member

Abdul Malik, Ph.D.

Committee Member

Bill Baker, Ph.D.

Committee Member

Martin Muschol, Ph.D.


lipids, liposomes, size-exclusion chromatography, encapsulation efficiency


Liposomes are viable candidates for drug delivery vehicles due to their ability to protect loaded compounds, dampen side effects of drugs, and be delivered to target sites in the body. The amphipathic nature of these lipid vesicles makes them very customizable through careful selection of bilayer and aqueous core components as well as synthetic and downsizing methods. However, synthetic methods may result in unencapsulated compounds remaining present alongside loaded liposomes, which require removal before the liposome suspension can be used.

This work explores the use of model DOPC as well as DOPC and CHOL liposomes and the methods that were optimized to produce, load, and purify them. Thin film hydration and extrusion were used to make and load the 50-200 nm sized vesicles. All liposomes were characterized using DLS, and loaded liposomes were successfully separated from free Cyt c via size-exclusion chromatography with specified parameters. Additional optimization is still needed to disrupt the liposomes for encapsulation efficiency determination as well as characterization of fluorescently dyed liposomes for future studies.

Included in

Chemistry Commons