Graduation Year
2023
Document Type
Thesis
Degree
M.S.
Degree Name
Master of Science (M.S.)
Degree Granting Department
Chemistry
Major Professor
Theresa Evans-Nguyen, Ph.D.
Committee Member
Abdul Malik, Ph.D.
Committee Member
Bill Baker, Ph.D.
Committee Member
Martin Muschol, Ph.D.
Keywords
lipids, liposomes, size-exclusion chromatography, encapsulation efficiency
Abstract
Liposomes are viable candidates for drug delivery vehicles due to their ability to protect loaded compounds, dampen side effects of drugs, and be delivered to target sites in the body. The amphipathic nature of these lipid vesicles makes them very customizable through careful selection of bilayer and aqueous core components as well as synthetic and downsizing methods. However, synthetic methods may result in unencapsulated compounds remaining present alongside loaded liposomes, which require removal before the liposome suspension can be used.
This work explores the use of model DOPC as well as DOPC and CHOL liposomes and the methods that were optimized to produce, load, and purify them. Thin film hydration and extrusion were used to make and load the 50-200 nm sized vesicles. All liposomes were characterized using DLS, and loaded liposomes were successfully separated from free Cyt c via size-exclusion chromatography with specified parameters. Additional optimization is still needed to disrupt the liposomes for encapsulation efficiency determination as well as characterization of fluorescently dyed liposomes for future studies.
Scholar Commons Citation
Khalife, Sandra, "Synthesis, Characterization, and Separation of Loaded Liposomes for Drug Delivery" (2023). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/9979
