Graduation Year
2019
Document Type
Dissertation
Degree
Ph.D.
Degree Name
Doctor of Philosophy (Ph.D.)
Degree Granting Department
Chemistry
Major Professor
Mildred Acevedo-Duncan, Ph.D.
Committee Member
Wayne C. Guida, Ph.D.
Committee Member
Jainfeng Cai, Ph.D.
Committee Member
Meera Nanjundan, Ph.D.
Committee Member
Marie Bourgeois, Ph.D.
Keywords
cell viability, apoptosis, ubiquitination, invasion
Abstract
Lung cancer is the second most common cancer, and it is the leading cause of cancer death in both men and women. Elevated levels of PKC-ι and FAK are implicated in the progression of lung cancer and involved in cell proliferation, apoptosis, cell survival, and cell migration processes. Lung cancer diagnosis typically occurs in the late stages, which limits the treatment options. Hence, there is a dire need to develop drugs that are effective and safe to treat lung cancers. We used a novel aPKC inhibitor named DNDA (3,4-amino-2,7 napthalene disulfonic acid) to study the role of atypical PKCs (aPKCs) in cell proliferation and migration in lung cancer cell lines. Our study demonstrates that DNDA reduced the cell viability, induced apoptosis and inhibited the migration of A549 lung cancer cells by PKC-ι/FAK ubiquitination via Cbl-b pathway. Hence, DNDA is an excellent candidate to treat lung cancers.
Scholar Commons Citation
Bommareddy, Raja Reddy, "Effect of Atypical protein kinase C inhibitor (DNDA) on Cell Proliferation and Migration of Lung Cancer Cells" (2019). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/8621
