Quantitative Proteomic Methodology Use and Development to Characterize Ethanol Modulation of Microglial Function
Graduation Year
2014
Document Type
Dissertation
Degree
Ph.D.
Degree Name
Doctor of Philosophy (Ph.D.)
Degree Granting Department
Cell Biology, Microbiology & Molecular Biology
Major Professor
Stanley M. Stevens Jr., Ph.D.
Co-Major Professor
Bin Liu, Ph.D.
Committee Member
Bin Liu, Ph.D.
Committee Member
Patrick Bradshaw, Ph.D.
Committee Member
Brant Burkhardt, Ph.D.
Keywords
Ethanol, Mass Spectrometry, Microglia, Neuroinflammation, Quantitative Proteomics
Abstract
Microglia act as the frontline immune defense in the brain. Microglial responses can be either neurotoxic, through the release of reactive oxygen and nitrogen species and inflammatory cytokines, or neurotrophic. Microglial activation due to chronic ethanol exposure has been implicated in neuroinflammation. We use mass spectrometric metabolic labeling techniques to explore and quantify the microglial proteome in immortalized cell lines and in vivo enriched microglia. Our proteomic profiling and subsequent validation suggests that microglia do activate in response to ethanol exposure, but the activation falls short of the classical, or M1 state of inflammatory activation, as no downstream markers for reactive species nor inflammatory cytokines can be found. Additionally, proteomic profiling suggests a partial activation marked by increased cell engulfment and cell movement in addition to increased release of inf-gamma and tgf-beta.
Scholar Commons Citation
Bell-Temin, Harris Benjamin, "Quantitative Proteomic Methodology Use and Development to Characterize Ethanol Modulation of Microglial Function" (2014). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/5620
