Graduation Year
2013
Document Type
Thesis
Degree
M.S.P.H.
Degree Granting Department
Global Health
Major Professor
Anthony Griffiths
Co-Major Professor
Holly Hughes
Keywords
evolution, Flavivirus, phylogeny, sequencing, West Nile virus
Abstract
The study of the evolution and phylogeny of West Nile virus (WNV) has been an important area of research since the introduction of WNV in 1999. However, genome sequencing of isolates from the Southeastern part of the United States has been somewhat limited. To determine how WNV has evolved at a more localized level, ten isolates from Florida and Georgia from 2003-2012 were completely sequenced using Illumina's next-generation technology. In addition, a phylogenetic comparison of both the complete genome and select partial genomes was completed to ensure consistency among the results. This study further demonstrated the dominance of the North American WN02 genotype within the Southeastern United States. In addition, phylogenetic analyses revealed the continued presence of genetic variance in 2012 with the finding of a new group within the North American clade. In conclusion, WNV has continued to evolve within the Southeastern US.
Scholar Commons Citation
Wedin, Crystal, "Complete Genome Sequences and Phylogeny of West Nile Virus Isolates from Southeastern United States, 2003-2012" (2013). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/4958
