Graduation Year
2005
Document Type
Dissertation
Degree
Ph.D.
Degree Granting Department
Biochemistry and Molecular Biology
Major Professor
William G. Kerr, Ph.D.
Committee Member
Larry P. Solomonson, Ph.D.
Committee Member
W. Douglas Cress, Ph.D.
Committee Member
Jiandong Chen, Ph.D.
Committee Member
Hong-Gang Wang, Ph.D.
Keywords
p53, E2F, EGFR, RNAi, AKT
Abstract
LRBA (LPS Responsive Beige-like Protein Kinase A anchor) gene expression is induced by the mitogen LPS and is a member of the WBW gene family member which is comprised of genes that are involved in cellular proliferation and differentiation. This work provides evidence for the over-expression of LRBA in certain cancers, and that LRBA promoter activity and endogenous LRBA mRNA levels are negatively regulated by the tumor suppressor p53 and positively regulated by E2F transactivators. Furthermore, we demonstrate that inhibition of LRBA expression or function leads to decreased proliferation of cancer cells and that LRBA plays a role in the EGFR signal transduction pathway. In addition to the findings of LRBA’s role in carcinogenesis, this work also shows evidence of the knockdown of the SH2-containing Inositol 5’ Phosphatase (SHIP) in both mouse and human cells. Furthermore, we provide evidence that SHIP-1 is involved in the AKT signal transduction pathway in human Natural Killer cells.
Scholar Commons Citation
Gamsby, Joshua John, "Study of the Roles of LRBA in Cancer Cell Proliferation and SHIP-1 in NK Cell Function" (2005). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/2886
