Graduation Year
2004
Document Type
Thesis
Degree
M.S.
Degree Granting Department
Physics
Major Professor
Myung K. Kim, Ph.D.
Committee Member
Wei Chen, Ph.D.
Committee Member
Dennis Killinger, Ph.D.
Keywords
biophysics, optics, hologram, microscope, cells
Abstract
We apply the techniques of digital holography to obtain microscopic 3D images of biological cells. Both the amplitude and phase images are obtained from a single hologram, with approximately 1 [mu]m lateral and 10 nm longitudinal resolution. The results are combined with previous experiments resolving the 2 [pi] ambiguity to produce continuous phase images of the samples.
Scholar Commons Citation
Parshall, Daniel, "Phase Imaging Digital Holography for Biological Microscopy" (2004). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/1191
