Graduation Year
2022
Document Type
Thesis
Degree
M.S.
Degree Name
Master of Science (M.S.)
Degree Granting Department
Chemistry
Major Professor
Subhra Mohapatra, Ph.D.
Committee Member
Eleni Markoutsa, Ph.D.
Committee Member
Shyam Mohapatra, Ph.D.
Keywords
Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FTIR), 17CL1 Fibroblast Cell Lines, Green Fluorescent Proteins (GFP)
Abstract
Coronaviruses have recently been under significant scrutiny due to the ongoing covid-19 pandemic that began in Wuhan, China, and subsequently spread to the rest of the world. Chitosan is a common chemical from crustaceans that has several desirable properties due to being biodegradable, non-toxic, and, most importantly, cheap. It also exhibits anti-viral properties, which is the focus of these experiments. We tested the toxicity of the chitosan on cell lines to see how much chitosan the cells can tolerate. We hydrolyzed the chitosan in acid, and the chitosan fibrils added cell lines to see if the anti-viral properties increased in those batches compared to the original. Afterwards, we tested the different chitosan samples against the mouse hepatitis virus to limited success when hydrolyzed for less than an hour. The hydrolyzed samples showed some greater efficacy than the starting material at concentrations 4 and 40 µg/ml.
Scholar Commons Citation
Sharma, Krishna, "Effects of acid hydrolyzed chitosan derivatives on MHV infection" (2022). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/10355
