Document Type
Article
Publication Date
2017
Keywords
collagen, cell adhesion, nordihydroguaiaretic acid, tendon replacement, orthopedic tissue engineering
Digital Object Identifier (DOI)
https://doi.org/10.3934/bioeng.2017.2.300
Abstract
Fixation is required to reinforce reconstituted collagen for orthopedic bioprostheses such as tendon or ligament replacements. Previous studies have demonstrated that collagen fibers cross-linked by the biocompatible dicatechol nordihydroguaiaretic acid (NDGA) have mechanical strength comparable to native tendons. This work focuses on investigating fibroblast behavior on fibrillar and NDGA cross-linked type I collagen to determine if NDGA modulates cell adhesion, morphology, and migration. A spinning disk device that applies a range of hydrodynamic forces under uniform chemical conditions was employed to sensitively quantify cell adhesion strength, and a radial barrier removal assay was used to measure cell migration on films suitable for these quantitative in vitro assays. The compaction of collagen films, mediated by the drying and cross-linking fabrication process, suggests a less open organization compared to native fibrillar collagen that likely allowed the collagen to form more inter-chain bonds and chemical links with NDGA polymers. Fibroblasts strongly adhered to and migrated on native and NDGA cross-linked fibrillar collagen; however, NDGA modestly reduced cell spreading, adhesion strength and migration rate. Thus, it is hypothesized that NDGA cross-linking masked some adhesion receptor binding sites either physically, chemically, or both, thereby modulating adhesion and migration. This alteration in the cell-material interface is considered a minimal trade-off for the superior mechanical and compatibility properties of NDGA cross-linked collagen compared to other fixation approaches.
Rights Information
This work is licensed under a Creative Commons Attribution 4.0 License.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
AIMS Bioengineering, v. 4, issue 2, p. 300-317
Scholar Commons Citation
Rioja, Ana Y.; Muniz-Maisonet, Maritza; Koob, Thomas J.; and Gallant, Nathan D., "Effect of Nordihydroguaiaretic Acid Cross-linking on Fibrillar Collagen: in Vitro Evaluation of Fibroblast Adhesion Strength and Migration" (2017). Chemical, Biological and Materials Engineering Faculty Publications. 9.
https://digitalcommons.usf.edu/ech_facpub/9
