Subcellular and in-vivo Nano-Endoscopy

Authors

Surya Venkatasekhar Cheemalapati, University of South Florida
John Winskas, University of South Florida
Hao Wang, University of South Florida
Karthik Konnaiyan, University of South Florida
Arseny Zhdanov, University of South Florida
Alison Roth, University of South Florida
Swamy Rakesh Adapa, University of South Florida
Andrew Deonarine, University of South Florida
Rays H. Y. Jiang, University of South Florida
Anna Pyayt, University of South FloridaFollow

Document Type

Article

Publication Date

10-3-2016

Keywords

Cellular Imaging, Mitochondria

Digital Object Identifier (DOI)

https://doi.org/10.1038/srep34400

Abstract

Analysis of individual cells at the subcellular level is important for understanding diseases and accelerating drug discovery. Nanoscale endoscopes allow minimally invasive probing of individual cell interiors. Several such instruments have been presented previously, but they are either too complex to fabricate or require sophisticated external detectors because of low signal collection efficiency. Here we present a nanoendoscope that can locally excite fluorescence in labelled cell organelles and collect the emitted signal for spectral analysis. Finite Difference Time Domain (FDTD) simulations have shown that with an optimized nanoendoscope taper profile, the light emission and collection was localized within ~100 nm. This allows signal detection to be used for nano-photonic sensing of the proximity of fluorophores. Upon insertion into the individual organelles of living cells, the nanoendoscope was fabricated and resultant fluorescent signals collected. This included the signal collection from the nucleus of Acridine orange labelled human fibroblast cells, the nucleus of Hoechst stained live liver cells and the mitochondria of MitoTracker Red labelled MDA-MB-231 cells. The endoscope was also inserted into a live organism, the yellow fluorescent protein producing nematode Caenorhabditis elegans, and a fluorescent signal was collected. To our knowledge this is the first demonstration of in vivo, local fluorescence signal collection on the sub-organelle level.

Comments

Complete list of authors:

Surya Venkatasekhar Cheemalapati, John Winskas, Hao Wang, Karthik Konnaiyan, Arseny Zhdanov, Alison Roth, Swamy Rakesh Adapa, Andrew Deonarine, Mark Noble, Tuhin Das, Robert Gatenby, Sandy D. Westerheide, Rays H. Y. Jiang, & Anna Pyayt

Rights Information

This work is licensed under a Creative Commons Attribution 4.0 License.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

Scientific Reports, v. 6, art. 3440

Scholar Commons Citation

Cheemalapati, Surya Venkatasekhar; Winskas, John; Wang, Hao; Konnaiyan, Karthik; Zhdanov, Arseny; Roth, Alison; Adapa, Swamy Rakesh; Deonarine, Andrew; Jiang, Rays H. Y.; and Pyayt, Anna, "Subcellular and in-vivo Nano-Endoscopy" (2016). Chemical, Biological and Materials Engineering Faculty Publications. 3.
https://digitalcommons.usf.edu/ech_facpub/3