This dataset contains a histological examination of livers of golden tilefish (Lopholatilus chamaeleonticeps) collected aboard the R/V Weatherbird II cruises WB-1304, WB-1403, WB-1502, WB-1602, WB-1603, and WB-1701 in the Gulf of Mexico from 2012-08-14 to 2016-09-06. The dataset includes the identified presence of histological changes and images of the gross and histological evaluations. Formalin-fixed tissues were individually evaluated for color, texture, size, shape, and consistency. Photographs were taken with a Sony-Cybershot camera (Sony Corporation, Minato, Tokyo, Japan). At least two photographs were taken of the samples from each container. The first photo contained the label along with the tissues from the sample jar. A second photo was taken of the samples to highlight gross morphology. If an abnormality was observed, additional photos were procured of the abnormality. Histological sections were stained with routine Hematoxylin and Eosin. Slides were evaluated blind in respect to the location and date of collection. After a preliminary evaluation, several fish were chosen to characterize specific lesions with special stains. Special stains included Periodic Acid-Schiff (PAS) with and without diastase, Gram stain, Perl’s Iron, Von Kossa, and Masson’s trichrome. Microscopic examination was performed using a Nikon Eclipse 80i (Nikon, Minato, Tokyo, Japan) and photomicrographs were taken with an Accu-scope Excelis HD (Commack, NY). The cruise documentation was provided for the R/V Weatherbird II cruises WB-1304, WB-1403, WB-1502, WB-1602, WB-1603, and WB-1701 led by chief scientists Dr. Steven Murawski and Dr. David Hollander. This dataset supports the publication: Pulster, E. L., Gracia, A., Snyder, S. M., Deak, K., Fogelson, S., & Murawski, S. A. (2019). Chronic Sub-lethal Effects Observed in Wild-Caught Fishes Following Two Major Oil Spills in the Gulf of Mexico: Deepwater Horizon and Ixtoc 1. Deep Oil Spills, 388–413. doi:10.1007/978-3-030-11605-7_24


Data and metadata is made available by the Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC) through a CC0 license in compliance with the Gulf of Mexico Research Initiative (GoMRI). The original dataset landing pages may be accessed at GRIIDC’s dataset monitoring webpage.

Data users are encouraged to contact the originating investigator prior to data use and provide appropriate credit.


Examine normal golden tilefish liver anatomy and the prevalence and frequency of microscopic hepatocellular changes.


golden tilefish, liver, histology, pathology, lesion, hepatocyte, microscopic change, fish examination, Lopholatilus chamaeleonticeps, Gross tissue evaluation





Point of Contact

Pulster, Erin
University of South Florida
College of Marine Science
140 7th Ave South
St. Petersburg , FL 33701

Funding Source


Start of Data Collection

August 2012

End of Data Collection

September 2016



Rights Information

Creative Commons License
This work is licensed under a Creative Commons Public Domain Dedication 1.0 License.