Effect of Agrochemical Exposure on Schistosoma Mansoni Cercariae Survival and Activity
Document Type
Article
Publication Date
2020
Keywords
Ecotoxicology, Parasite, Organophosphate, Pyrethroid, Trematode
Digital Object Identifier (DOI)
https://doi.org/10.1002/etc.4732
Abstract
Singular use of activity assays or staining dyes to assess pathogen agrochemical tolerance can underestimate tolerance if pesticides cause sublethal effects. We exposed Schistosoma mansoni cercariae, the aquatic life stage of this trematode that infects humans, to 4 insecticides at 5 concentrations using a 24-h time-to-death assay. We used Trypan blue dye, which stains dead tissue, and activity assays simultaneously to discriminate dead from live but paralyzed individuals. Whereas cypermethrin, deltamethrin, and dimethoate exposure did not affect cercariae at any ecologically relevant concentrations, methamidophos exposure increased survival of cercariae compared with those in the controls. This was because methamidophos-induced paralysis reduced cercarial activity and thus energy expenditures, extending the lifespan of this short-lived parasite that causes human schistosomiasis. These findings highlight that sublethal effects should be considered when pesticide effects on disease are under investigation. Environ Toxicol Chem 2020;39:1421–1428. © 2020 SETAC
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Environmental Toxicology and Chemistry, v. 39, issue 7, p. 1421-1428
Scholar Commons Citation
Jones, Devin K.; Davila, David D.; Nguyen, Karena H.; and Rohr, Jason R., "Effect of Agrochemical Exposure on Schistosoma Mansoni Cercariae Survival and Activity" (2020). Integrative Biology Faculty and Staff Publications. 558.
https://digitalcommons.usf.edu/bin_facpub/558
