Ubiquity and Functional Uniformity in CO2 Concentrating Mechanisms in Multiple Phyla of Bacteria is Suggested by a Diversity and Prevalence of Genes Encoding Candidate Dissolved Inorganic Carbon Transporters

Authors

Kathleen Scott, University of South FloridaFollow
Tara L. Harmer, Stockton University
Bradford Gemmell, University of South FloridaFollow
Andrew M. Kramer, University of South FloridaFollow
Markus Sutter, Lawrence Berkeley National Laboratory
Cheryl A. Kerfeld, Lawrence Berkeley National Laboratory
Kourtney S. Barber, University of South Florida
Saaurav Bari, University of South Florida
Joshua W. Boling, University of South Florida
Cassandra P. Campbell, University of South Florida
Javier F. Gallard-Gongora, University of South Florida
Jessica K. Jackson, University of South Florida
Aldo Lobos, University of South Florida
Jeannie M. Mounger, University of South Florida
Peter W. Radulovic, University of South Florida
Jacqueline M. Sanson, University of South Florida
Sarah Schmid, University of South Florida
Candice Takieddine, University of South Florida
Kiley F. Warlick, University of South Florida
Robert Whittaker, University of South Florida

Document Type

Article

Publication Date

2020

Keywords

CO2 concentrating mechanism, autotroph, carbon fixation, carboxysome

Digital Object Identifier (DOI)

https://doi.org/10.1093/femsle/fnaa106

Abstract

Autotrophic microorganisms catalyze the entry of dissolved inorganic carbon (DIC; = CO₂ + HCO₃− + CO₃2−) into the biological component of the global carbon cycle, despite dramatic differences in DIC abundance and composition in their sometimes extreme environments. “Cyanobacteria” are known to have CO₂ concentrating mechanisms (CCMs) to facilitate growth under low CO₂ conditions. These CCMs consist of carboxysomes, containing enzymes ribulose 1,5-bisphosphate oxygenase and carbonic anhydrase, partnered to DIC transporters. CCMs and their DIC transporters have been studied in a handful of other prokaryotes, but it was not known how common CCMs were beyond “Cyanobacteria”. Since it had previously been noted that genes encoding potential transporters were found neighboring carboxysome loci, α-carboxysome loci were gathered from bacterial genomes, and potential transporter genes neighboring these loci are described here. Members of transporter families whose members all transport DIC (CHC, MDT and Sbt) were common in these neighborhoods, as were members of the SulP transporter family, many of which transport DIC. 109 of 115 taxa with carboxysome loci have some form of DIC transporter encoded in their genomes, suggesting that CCMs consisting of carboxysomes and DIC transporters are widespread not only among “Cyanobacteria”, but also among members of “Proteobacteria” and “Actinobacteria”.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

Microbiology Letters, v. 367, issue 13, art. fnaa106

Scholar Commons Citation

Scott, Kathleen; Harmer, Tara L.; Gemmell, Bradford; Kramer, Andrew M.; Sutter, Markus; Kerfeld, Cheryl A.; Barber, Kourtney S.; Bari, Saaurav; Boling, Joshua W.; Campbell, Cassandra P.; Gallard-Gongora, Javier F.; Jackson, Jessica K.; Lobos, Aldo; Mounger, Jeannie M.; Radulovic, Peter W.; Sanson, Jacqueline M.; Schmid, Sarah; Takieddine, Candice; Warlick, Kiley F.; and Whittaker, Robert, "Ubiquity and Functional Uniformity in CO2 Concentrating Mechanisms in Multiple Phyla of Bacteria is Suggested by a Diversity and Prevalence of Genes Encoding Candidate Dissolved Inorganic Carbon Transporters" (2020). Integrative Biology Faculty and Staff Publications. 491.
https://digitalcommons.usf.edu/bin_facpub/491