Electrofusion of Individual Animal Cells Directly to Intact Corneal Epithelial Tissue
Document Type
Article
Publication Date
3-27-1989
Keywords
Electrofusion, Cell hybrid, Membrane receptor transfer, (Human cell), (Rabbit tissue), (Somatic cell hybrid)
Digital Object Identifier (DOI)
https://doi.org/10.1016/0005-2736(89)90193-4
Abstract
An electromechanical process was developed to electrofuse human and nonhuman cultured cells directly to rabbit corneal epithelial tissue in vitro and in situ. This new process was utilized successfully to incorporate functional gonococcal membrane attachment receptors from human lymphoma cells into superficial rabbit corneal epithelium. Thus, cell-tissue electrofusion biotechnology may be employed to establish unique and novel animal models for investigating receptor-mediated processes in vivo.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Biochimica et Biophysica Acta (BBA) - Biomembranes, v. 980, issue 1, p. 9-14
Scholar Commons Citation
Grasso, Robert J.; Heller, Richard; Cooley, John C.; and Haller, Edward M., "Electrofusion of Individual Animal Cells Directly to Intact Corneal Epithelial Tissue" (1989). Integrative Biology Faculty and Staff Publications. 279.
https://digitalcommons.usf.edu/bin_facpub/279
