Document Type
Article
Publication Date
2018
Digital Object Identifier (DOI)
https://doi.org/10.1371/journal.pone.0200275
Abstract
Isogenic wild type yeast cells raised in controlled environments display a significant range of lifespan variation. Recent microfluidic studies suggest that differential growth or gene expression patterns may explain some of the heterogeneity of aging assays. Herein, we sought to complement this work by similarly examining a large set of replicative lifespan data from traditional plate assays. In so doing, we reproduced the finding that short-lived cells tend to arrest at senescence with a budded morphology. Further, we found that wild type cells born unusually small did not have an extended lifespan. However, large birth size and/or high inter-generational growth rates significantly correlated with a reduced lifespan. Finally, we found that SIR2 expression levels correlated with lifespan and intergenerational growth. SIR2 expression was significantly reduced in large cells and increased in small wild type cells. A moderate increase in SIR2 expression correlated with reduced growth, decreased proliferation and increased lifespan in plate aging assays. We conclude that cellular growth rates and SIR2 expression levels may contribute to lifespan variation in individual cells.
Rights Information
This work is licensed under a Creative Commons Attribution 4.0 License.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
PLoS ONE, v. 13, issue 7, art. e0200275
Scholar Commons Citation
Smith, Jessica T.; White, Jill W.; Dungrawala, Huzefa; Hua, Hui; and Schneider, Brandt L., "Yeast Lifespan Variation Correlates with Cell Growth and Sir2 Expression" (2018). Molecular Biosciences Faculty Publications. 98.
https://digitalcommons.usf.edu/bcm_facpub/98
