The MDMX Acidic Domain Uses Allovalency to Bind Both P53 and MDMX
Document Type
Article
Publication Date
2022
Keywords
Protein Disorder, P53 Mimicry, Autoinhibition, Allovalency
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.jmb.2022.167844
Abstract
Autoinhibition of p53 binding to MDMX requires two short-linear motifs (SLiMs) containing adjacent tryptophan (WW) and tryptophan-phenylalanine (WF) residues. NMR spectroscopy was used to show the WW and WF motifs directly compete for the p53 binding site on MDMX and circular dichroism spectroscopy was used to show the WW motif becomes helical when it is bound to the p53 binding domain (p53BD) of MDMX. Binding studies using isothermal titration calorimetry showed the WW motif is a stronger inhibitor of p53 binding than the WF motif when they are both tethered to p53BD by the natural disordered linker. We also investigated how the WW and WF motifs interact with the DNA binding domain (DBD) of p53. Both motifs bind independently to similar sites on DBD that overlap the DNA binding site. Taken together our work defines a model for complex formation between MDMX and p53 where a pair of disordered SLiMs bind overlapping sites on both proteins.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Journal of Molecular Biology, v. 434, issue 22, art. 167844
Scholar Commons Citation
Fenton, Malissa; Borcherds, Wade; Chen, Lihong; Anbanandam, Asokan; Levy, Robin; Chen, Jiandong; and Daughdrill, Gary W., "The MDMX Acidic Domain Uses Allovalency to Bind Both P53 and MDMX" (2022). Molecular Biosciences Faculty Publications. 141.
https://digitalcommons.usf.edu/bcm_facpub/141
