Tracking Cell Wall-anchored Proteins in Gram-positive Bacteria
Document Type
Book Chapter
Publication Date
2024
Keywords
Immunofluorescence Microscopy, Cell Wall-anchored Proteins, Staphylococcus Aureus, Protein A (Spa), Surface Display, Cross-wall, Septum
Digital Object Identifier (DOI)
https://doi.org/10.1007/978-1-0716-3491-2_15
Abstract
Cell wall-anchored surface proteins are integral components of Gram-positive bacterial cell envelope and vital for bacterial survival in different environmental niches. To fulfill their functions, surface protein precursors translocate from cytoplasm to bacterial cell surface in three sequential steps: secretion across the cytoplasmic membrane, covalently anchoring to the cell wall precursor lipid II by sortase A, and incorporation of the lipid II-linked precursors into mature cell wall peptidoglycan. Here, we describe a series of immunofluorescence microscopy methods to track the subcellular localization of cell wall-anchored proteins along the sorting pathway. While the protocols are tailored to Staphylococcus aureus, they can be readily adapted to localize cell wall-anchored proteins as well as membrane proteins in other Gram-positive bacteria.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Tracking Cell Wall-anchored Proteins in Gram-positive Bacteria, in Hung Ton-That (Ed.), The Bacterial Cell Wall, v. 2727, Humana, p. 193-204
Scholar Commons Citation
Scaffidi, Salvatore J. and Yu, Wenqi, "Tracking Cell Wall-anchored Proteins in Gram-positive Bacteria" (2024). Molecular Biosciences Faculty Publications. 137.
https://digitalcommons.usf.edu/bcm_facpub/137
