Synchronization of Yeast
Document Type
Book Chapter
Publication Date
2017
Keywords
Yeast, Cell Cycle, Synchronization, Block-and-release, Centrifugal Elutriation
Digital Object Identifier (DOI)
https://doi.org/10.1007/978-1-4939-6603-5_14
Abstract
The budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe are amongst the simplest and most powerful model systems for studying the genetics of cell cycle control. Because yeast grows very rapidly in a simple and economical media, large numbers of cells can easily be obtained for genetic, molecular, and biochemical studies of the cell cycle. The use of synchronized cultures greatly aids in the ease and interpretation of cell cycle studies. In principle, there are two general methods for obtaining synchronized yeast populations. Block-and-release methods can be used to induce cell cycle synchrony. Alternatively, centrifugal elutriation can be used to select synchronous populations. Because each method has innate advantages and disadvantages, the use of multiple approaches helps in generalizing results. An overview of the most commonly used methods to generate synchronized yeast cultures is presented along with working Notes: a section that includes practical comments, experimental considerations and observations, and hints regarding the pros and cons innate to each approach.
Citation / Publisher Attribution
Synchronization of Yeast, in G. Banfalvi (Ed.), Cell Cycle Synchronization, v. 1524, Humana Press, p. 215-242
Scholar Commons Citation
Smith, Jessica; Manukyan, Arkadi; Hua, Hui; Dungrawala, Huzefa; and Schneider, Brandt L., "Synchronization of Yeast" (2017). Molecular Biosciences Faculty Publications. 113.
https://digitalcommons.usf.edu/bcm_facpub/113
