The electroporation system and method combine pulses having different characteristics for delivering molecules to cells in vivo. The pulses include a high-intensity pulse for inducing electroporation and a low-intensity pulse to induce electrophoretic molecule movement within an interstitial space, molecule adherence to a cell membrane, and electrophoretic movement of the molecule through the permeabilized membrane. The use of a high-intensity and a low-intensity pulse achieves improved delivery; reduction of intensity and/or duration of pulses for inducing electroporation; and decreased muscle stimulation, tissue damage, and patient discomfort.
Heller, Richard; Gilbert, Richard; Jaroszeski, Mark J.; Heller, Loree C.; and Lucas, Melinda L., "Electroporation and electrophoresis system and method for achieving molecular penetration into cells in vivo" (2004). USF Patents. 751.
University of South Florida