Poster Preview
Title: Exploring Marine Microbial Products: Targeting Medical Bacteria in Little Bayou Bay.
Abstract:
This study focuses on the identification and curation of microbial natural products, with a specific interest in their potential to target medical-related bacteria. The environment of the Little Bayou Bay was sandy with dirt near alley water that goes out to the Little Bayou Bay. The depth of sample was collected can have different pH level, and bacteria that live in the area. Understanding these reasons why microbial natural target medical-related bacteria can open numerous treatment options. The goal of the research lab is to isolate a bacterium from a sample from Little Bayou Bay. During the lab conduct multiple experiment that include high-density bacterial plating, compare isolation to killer cells, Kirby-Baur test, and multiple test that help determine if the bacteria is positive or negative. Following the Kirby-Bauer test, the bacterial isolates BAOB-4 and BAOB-5 demonstrated promising antimicrobial activity against Staphylococcus aureus. Through 16S rRNA sequencing, these isolates were identified as belonging to the genus and species [Bacillus and Bacillus stratosphericus]. The identification of BAOB-4 was unsuccessful because of an error that occurred during the 16S PCR sequencing process. The sample will be resent for identification. The study establishes a foundation for further exploration into marine natural products and their application in hoping it could be use in helping target cancer cells in the future.
Keyword: Little Bayou Bay's, targets medical bacteria, Bacillus stratosphericus, Marine Microbial Products
College
College of Arts and Sciences
Mentor Information
Michael Shamblott
Description
This study focuses on the identification and curation of microbial natural products, with a specific interest in their potential to target medical-related bacteria. The environment of the Little Bayou Bay was sandy with dirt near alley water that goes out to the Little Bayou Bay. The depth of sample was collected can have different pH level, and bacteria that live in the area. Understanding these reasons why microbial natural target medical-related bacteria can open numerous treatment options. The goal of the research lab is to isolate a bacterium from a sample from Little Bayou Bay. During the lab conduct multiple experiment that include high-density bacterial plating, compare isolation to killer cells, Kirby-Baur test, and multiple test that help determine if the bacteria is positive or negative. Following the Kirby-Bauer test, the bacterial isolates BAOB-4 and BAOB-5 demonstrated promising antimicrobial activity against Staphylococcus aureus. Through 16S rRNA sequencing, these isolates were identified as belonging to the genus and species [Bacillus and Bacillus stratosphericus]. The identification of BAOB-4 was unsuccessful because of an error that occurred during the 16S PCR sequencing process. The sample will be resent for identification. The study establishes a foundation for further exploration into marine natural products and their application in hoping it could be use in helping target cancer cells in the future.
Exploring Marine Microbial Products: Targeting Medical Bacteria in Little Bayou Bay
This study focuses on the identification and curation of microbial natural products, with a specific interest in their potential to target medical-related bacteria. The environment of the Little Bayou Bay was sandy with dirt near alley water that goes out to the Little Bayou Bay. The depth of sample was collected can have different pH level, and bacteria that live in the area. Understanding these reasons why microbial natural target medical-related bacteria can open numerous treatment options. The goal of the research lab is to isolate a bacterium from a sample from Little Bayou Bay. During the lab conduct multiple experiment that include high-density bacterial plating, compare isolation to killer cells, Kirby-Baur test, and multiple test that help determine if the bacteria is positive or negative. Following the Kirby-Bauer test, the bacterial isolates BAOB-4 and BAOB-5 demonstrated promising antimicrobial activity against Staphylococcus aureus. Through 16S rRNA sequencing, these isolates were identified as belonging to the genus and species [Bacillus and Bacillus stratosphericus]. The identification of BAOB-4 was unsuccessful because of an error that occurred during the 16S PCR sequencing process. The sample will be resent for identification. The study establishes a foundation for further exploration into marine natural products and their application in hoping it could be use in helping target cancer cells in the future.
