The Effect of Iron Loading on Hepcidin
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Mentor Information
Dr Thao Ho
Description
Regulating iron is important because low or high iron levels can lead to improper functioning of the body system Hepcidin produced by the liver controls the level of iron in circulation by preventing iron release from cells Preterm infants are at risk for iron deficiency and they are given oral iron supplement to prevent it Iron is released from the duodenal enterocytes into circulation via iron transporters called ferroportin which is controlled by hepcidin This study aims to understand how iron dose administriation influences serum hepcidin levels Because it is a challenge to obtain repeated blood sampling in preterm infants, we first demonstrated urine hepcidin correlated with serum hepcidin using remnant blood samples (n=79, Spearman r=0 437, <0 05) Hepcidin levels in 79 serum samples and 79 urine samples were measured using the Intrinsic Hepcidin IDx™ ELISA kit For urine samples, the creatinine levels were also measured using the Creatinine Parameter Assay Kit for normalization This indicates that urine hepcidin levels can represent the changes in serum hepcidin Then we showed that urine hepcidin levels after an iron loading were significantly higher than the hepcidin level prior to the iron administration (n=9, paired student t, <0 05) This suggests that the frequency of iron dosing can affect iron absorption via hepcidin production Further research on the iron regulatory mechanism in preterm infants is needed
The Effect of Iron Loading on Hepcidin
Regulating iron is important because low or high iron levels can lead to improper functioning of the body system Hepcidin produced by the liver controls the level of iron in circulation by preventing iron release from cells Preterm infants are at risk for iron deficiency and they are given oral iron supplement to prevent it Iron is released from the duodenal enterocytes into circulation via iron transporters called ferroportin which is controlled by hepcidin This study aims to understand how iron dose administriation influences serum hepcidin levels Because it is a challenge to obtain repeated blood sampling in preterm infants, we first demonstrated urine hepcidin correlated with serum hepcidin using remnant blood samples (n=79, Spearman r=0 437, <0 >05) Hepcidin levels in 79 serum samples and 79 urine samples were measured using the Intrinsic Hepcidin IDx™ ELISA kit For urine samples, the creatinine levels were also measured using the Creatinine Parameter Assay Kit for normalization This indicates that urine hepcidin levels can represent the changes in serum hepcidin Then we showed that urine hepcidin levels after an iron loading were significantly higher than the hepcidin level prior to the iron administration (n=9, paired student t, <0 >05) This suggests that the frequency of iron dosing can affect iron absorption via hepcidin production Further research on the iron regulatory mechanism in preterm infants is needed
