Candida and Bioactive Compounds in Penicillium Purporogenum
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Mentor Information
Dr. Bill Baker
Description
Throughout many areas of medicine, there has been a prevalent rise in multidrug-resistant infections. This poses a significant problem for many individuals, especially those who are immunocompromised. One such infection is the prevalence of Candida, a fungus that can cause thrush and yeast infection. Through the exploration of bioactive compounds found in fungal samples, we can find alternative pathways through compound isolation to treat such infections. This research seeks to explore the bioactive compounds found in Penicillium purporogenum, their resistance to the fungal species Candida, and to further isolate compounds. To accomplish this, the methods used included rice cultivation, extraction, MPLC and HPLC purification, bioassay testing, mass spectrometry, and nuclear magnetic resonance spectroscopy. The fungus is first grown with three different epigenetic modifiers (DNA Methyltransferase, Nicotinamide, Butyrate) along with a control. Compounds are then isolated using partitions to eliminate primary metabolites, then further isolated using MPLC and HPLC purification. The subsequent samples are then run through bioassay testing to determine if they have any potential effect on Candida. The bioactive compounds are then run through mass spectrometry and nuclear magnetic resonance spectroscopy to determine their structure. The isolation of these known and unknown compounds will contribute to the research on Penicillium purporogenum and potential uses against Candida.
Candida and Bioactive Compounds in Penicillium Purporogenum
Throughout many areas of medicine, there has been a prevalent rise in multidrug-resistant infections. This poses a significant problem for many individuals, especially those who are immunocompromised. One such infection is the prevalence of Candida, a fungus that can cause thrush and yeast infection. Through the exploration of bioactive compounds found in fungal samples, we can find alternative pathways through compound isolation to treat such infections. This research seeks to explore the bioactive compounds found in Penicillium purporogenum, their resistance to the fungal species Candida, and to further isolate compounds. To accomplish this, the methods used included rice cultivation, extraction, MPLC and HPLC purification, bioassay testing, mass spectrometry, and nuclear magnetic resonance spectroscopy. The fungus is first grown with three different epigenetic modifiers (DNA Methyltransferase, Nicotinamide, Butyrate) along with a control. Compounds are then isolated using partitions to eliminate primary metabolites, then further isolated using MPLC and HPLC purification. The subsequent samples are then run through bioassay testing to determine if they have any potential effect on Candida. The bioactive compounds are then run through mass spectrometry and nuclear magnetic resonance spectroscopy to determine their structure. The isolation of these known and unknown compounds will contribute to the research on Penicillium purporogenum and potential uses against Candida.
