Isolation and Characterization of Novel Metabolites from the Antarctic Nudibranch Doris Kerguelenensis
Mentor Information
Bill Baker (Chemistry) & Sarah Dietrick (Chemistry)
Description
In recent years, natural product research has identified an Antarctic specimen, Doris kerguelenensis, to contain unique metabolites with remarkable pharmacological application. The chemodiversity of D. kerguelensis results from its exclusive nutrient intake and extreme habitat. One study uncovered palmadorin diterpenoids (novel compounds native to Doris kerguelenesis) to be effective inhibitors against Jak2/STAT5-dependent HEL leukemia cells1, thus possessing potential to aid in future drug development. Prompted by the pharmacological potential of palmadorin diterpenoids, further investigation of the chemodiverse D. kerguelenesis continues in pursuit of other metabolites that display bioactive potential against different human ailments, such as various cancers and Covid-19 proteins. Methods of investigation include targeted isolation and extraction from D. kerguelenesis samples, followed by analysis via NMR, MS, and HPLC. The characterization data is then used to compile a catalogue of the identified metabolites, their observed quantities, and the phylogroup of origin. This collection of data facilitates a dynamic comparison of phylogenetic and metabolomic properties -- a critical component in the pursuit to understand the full pharmacological potential of D. kerguelenensis. References: (1) Maschek, J. A.; Mevers, E.; Diyabalanage, T.; Chen, L.; Ren, Y.; McClintock, J. B.; Amsler, C. D.; Wu, J.; Baker, B. J. Palmadorin Chemodiversity from the Antarctic Nudibranch Austrodoris Kerguelenensis and Inhibition of Jak2/STAT5-Dependent HEL Leukemia Cells. Tetrahedron2012, 68(44), 9095–9104.
Isolation and Characterization of Novel Metabolites from the Antarctic Nudibranch Doris Kerguelenensis
In recent years, natural product research has identified an Antarctic specimen, Doris kerguelenensis, to contain unique metabolites with remarkable pharmacological application. The chemodiversity of D. kerguelensis results from its exclusive nutrient intake and extreme habitat. One study uncovered palmadorin diterpenoids (novel compounds native to Doris kerguelenesis) to be effective inhibitors against Jak2/STAT5-dependent HEL leukemia cells1, thus possessing potential to aid in future drug development. Prompted by the pharmacological potential of palmadorin diterpenoids, further investigation of the chemodiverse D. kerguelenesis continues in pursuit of other metabolites that display bioactive potential against different human ailments, such as various cancers and Covid-19 proteins. Methods of investigation include targeted isolation and extraction from D. kerguelenesis samples, followed by analysis via NMR, MS, and HPLC. The characterization data is then used to compile a catalogue of the identified metabolites, their observed quantities, and the phylogroup of origin. This collection of data facilitates a dynamic comparison of phylogenetic and metabolomic properties -- a critical component in the pursuit to understand the full pharmacological potential of D. kerguelenensis. References: (1) Maschek, J. A.; Mevers, E.; Diyabalanage, T.; Chen, L.; Ren, Y.; McClintock, J. B.; Amsler, C. D.; Wu, J.; Baker, B. J. Palmadorin Chemodiversity from the Antarctic Nudibranch Austrodoris Kerguelenensis and Inhibition of Jak2/STAT5-Dependent HEL Leukemia Cells. Tetrahedron2012, 68(44), 9095–9104.
