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Sk Sarif Hassan, Pingla Thana Mahavidyalaya
Alaa A. A. Aljabali, Yarmouk University-Faculty of Pharmacy
Pritam Kumar Panda, Uppsala University
Shinjini Ghosh, University of Calcutta
Diksha Attrish, University of Delhi
Pabitra Pal Choudhury, Indian Statistical Institute, Kolkata
Murat Seyran, University of Vienna
Damiano Pizzol, Italian Agency for Development Cooperation - Khartoum
Parise Adadi, University of Otago, Dunedin
Tarek Mohamed Abd El-Aziz, Minia University
Antonio Soares, University of Texas Health Science Center at San Antonio
Ramesh Kandimalla, CSIR-Indian Institute of Chemical Technology Uppal Road
Kenneth Lundstrom, PanTherapeutics, Rte de Lavaux 49, CH1095, Lutry
Amos Lal, Mayo Clinic, Rochester
Gajendra Kumar Azad, Patna University
Vladimir N. Uversky, University of South FloridaFollow
Samendra P. Sherchan, Tulane University
Wagner Baetas-da-Cruz, Federal University of Rio de Janeiro (UFRJ)
Bruce D. Uhal, Michigan State University
Nima Rezaei, Tehran University of Medical Sciences
Gaurav Chauhan, School of Engineering and Sciences, Tecnologico de Monterrey
Debmalya Barh, Institute of Integrative Omics and Applied Biotechnology (IIOAB), PatnaPatna
Elrashdy M. Redwan, King Abdulazizi University
Guy W. Dayhoff, University of South Florida
Nicolas G. Bazan, Louisiana State University Health New Orleans
Ángel Serrano-Aroca, Catholic University of Valencia San Vicente Mártir
Amr El-Demerdash, Institute de Chimie des Substances Naturelles
Yogendra K. Mishra, University of Southern Denmark
Giorgio Palu, University of Padova
Kazuo Takayama, Kyoto University
Adam M. Brufsky, University of Pittsburgh School of Medicine
Murtaza M. Tambuwala, Ulster University

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ORF8, Phylogenetics, Mutational hotspots, Physicochemical properties, ORF8 evolution, SARS-CoV-2



Immune evasion is one of the unique characteristics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) attributed to its ORF8 protein. This protein modulates the adaptive host immunity through down-regulation of MHC-1 (Major Histocompatibility Complex) molecules and innate immune responses by surpassing the host's interferon-mediated antiviral response. To understand the host's immune perspective in reference to the ORF8 protein, a comprehensive study of the ORF8 protein and mutations possessed by it have been performed. Chemical and structural properties of ORF8 proteins from different hosts, such as human, bat, and pangolin, suggest that the ORF8 of SARS-CoV-2 is much closer to ORF8 of Bat RaTG13-CoV than to that of Pangolin-CoV. Eighty-seven mutations across unique variants of ORF8 in SARS-CoV-2 can be grouped into four classes based on their predicted effects (Hussain et al., 2021) [1]. Based on the geo-locations and timescale of sample collection, a possible flow of mutations was built. Furthermore, conclusive flows of amalgamation of mutations were found upon sequence similarity analyses and consideration of the amino acid conservation phylogenies. Therefore, this study seeks to highlight the uniqueness of the rapidly evolving SARS-CoV-2 through the ORF8.

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Computers in Biology and Medicine, v. 133, art. 104380