Screening Pathological Protein Specific Antibodies as Biomarker Discovery Tools for Neurodegenerative Disease
Master of Science (M.S.)
Degree Granting Department
Chuanhai Cao, Ph.D.
Umesh Jinwal, Ph.D.
Wanling Zhou, Ph.D.
Qingyu Xuan, Ph.D.
Alzheimer’s disease, beta-amyloid, Parkinson’s disease, α-synuclein
With the increase in the average life span, the incidence of neurodegenerative diseases also increases, among which Alzheimer's disease (AD) and Parkinson's disease (PD) are the two most common types of neurological disorders. AD and PD share the same pathogenic mechanism that involves a class of proteins called beta-amyloid (Aβ), which, under the induction of some factors, changes from its natural monomeric state to the oligomers containing β-sheet, which is neurotoxic and thus leads to cell damage or apoptosis. This study focused on the selection of antibodies used as biomarkers for AD and PD. The selection of antibodies was based on the two well-accepted biomarkers of AD and PD, i.e., Aβ proteins and α-synuclein (α-syn) Isothermal titration calorimetry (ITC) was used to determine the specific binding of individual antibodies to their target proteins.
Scholar Commons Citation
Li, Yitong, "Screening Pathological Protein Specific Antibodies as Biomarker Discovery Tools for Neurodegenerative Disease" (2022). USF Tampa Graduate Theses and Dissertations.