Graduation Year
2022
Document Type
Thesis
Degree
M.S.
Degree Name
Master of Science (M.S.)
Degree Granting Department
Chemistry
Major Professor
Bill Baker, Ph.D.
Committee Member
Theresa Evans-Nguyen, Ph.D.
Committee Member
Edward Turos, Ph.D.
Keywords
drug discovery, endophytic fungi, natural products, secondary metabolites
Abstract
Natural products have played major roles in drug discovery and provided sources for many new drugs. Secondary metabolites, also called natural products, are organic compounds that are produced from fungi, bacteria, and other living organisms. Secondary metabolites produced by endophytic fungi include alkaloids, phenols, peptides, enzymes, polyketides, and terpenes. Endophytic fungi are rich sources of secondary metabolites and demonstrate bioactivity against many human pathogens including the ESKAPE pathogens. This thesis explores the diverse roles of endophytic fungi and their production of unique secondary metabolites for potential drug discovery and development. Secondary metabolites from two endophytic fungi (EG10-21E-2-HDAC & TAP14-34A-2-HDAC), collected from Mexico and Florida, were isolated and characterized using dereplication and compound isolation methods. Methods of dereplication using LC-MS/MS applied to the fungi resulted in identification of known toxins and diverse secondary metabolites using molecular networking. Isolated compounds were prioritized and characterized using NMR analysis and mass spectrometry techniques. Fungal identification data of EG10-21E-2 & TAP14-34A-2 were obtained following DNA extraction, PCR amplification, Sanger sequencing, and phylogenetic analyses.
Scholar Commons Citation
Thompson, Stephen, "Metabolomic Analysis, Identification and Antimicrobial Assay of Two Mangrove Endophytes" (2022). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/9481
