Graduation Year


Document Type




Degree Name

Master of Science (M.S.)

Degree Granting Department

Biology (Cell Biology, Microbiology, Molecular Biology)

Major Professor

James R. Garey, Ph.D.

Co-Major Professor

Alan R. Franck, Ph.D.

Committee Member

Diane Te Strake, Ph.D.


Boletaceae, Pulchroboletus, Pseudophylloporus, Systematics


The boletes are macrofungi which have undergone extensive taxonomic revisions since the advent of molecular tools. To further our understanding of the boletes in peninsular Florida, we sequenced two common Floridian boletes, and analyzed them with molecular phylogenetic tools. Boletus rubricitrinus, a common Florida bolete often found in lawns under Quercus, and likely has a distribution that extends to Texas. Based on ITS and LSU sequences and morphological studies, this species belongs in the genus Pulchroboletus. As the holotype is in poor condition, an epitype is established here. A thorough description of macroscopic and microscopic features is also provided for the species. Fungi in the genus Phylloporus are lamellate boletes that occur worldwide, but primarily in the tropics. Phylloporus boletinoides is a species which was described from Florida, and is found growing near Pinus spp. Based on ITS, LSU, and RPB1 sequences, we establish the novel genus Pseudophylloporus, which is allied to Bothia and Solioccasus. Morphological data are also provided from our collections, and one from Belize. Based on molecular data and a review of bolete literature, the delimitation of this genus suggests that there are three distinct lineages of boletes that have a lamellate hymenium in the Boletaceae. These molecular and morphological data will be useful to further improve our understanding of bolete taxonomy.