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Cadmium treatment induces oxidative phosphorylation (OXPHOS)

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Indu Parameswaran

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Tampa

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Narasaiah Kolliputi

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Introduction: Cadmium is a toxic pollutant, and humans are exposed to cadmium by smoking cigarettes. Its effect on mitochondrial OXPHOS is unknown in RAW cells.

Methods: RAW cells were exposed to various concentrations of CdCl2 for 24 h. Cell toxicity was determined. RAW cells were treated with CdCl2 (5µM, 10µM, 15µM) for 24h and subjected to Western blot using OXPHOS, HO-1, and SOD2. Flow cytometry was performed to assess pyroptosis.

Results: A concentration of 25µM Cd induced 50% of cell death. OXPHOS complexes (I-V) expression was higher in Cd-treated cells than in control. Flow cytometry analysis revealed an increase in pyroptosis in Cd-treated cells. SOD2, mitochondrial superoxide dismutase, expression increased with Cd treatment. Heme oxygenase 1 (HO-1) expression increased at low concentrations and decreased at higher concentrations of Cd.

Conclusion: The data suggest that Cd induces pyroptosis, oxidative stress and affects OXPHOS complex expression in RAW cells.

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Cadmium treatment induces oxidative phosphorylation (OXPHOS)

Introduction: Cadmium is a toxic pollutant, and humans are exposed to cadmium by smoking cigarettes. Its effect on mitochondrial OXPHOS is unknown in RAW cells.

Methods: RAW cells were exposed to various concentrations of CdCl2 for 24 h. Cell toxicity was determined. RAW cells were treated with CdCl2 (5µM, 10µM, 15µM) for 24h and subjected to Western blot using OXPHOS, HO-1, and SOD2. Flow cytometry was performed to assess pyroptosis.

Results: A concentration of 25µM Cd induced 50% of cell death. OXPHOS complexes (I-V) expression was higher in Cd-treated cells than in control. Flow cytometry analysis revealed an increase in pyroptosis in Cd-treated cells. SOD2, mitochondrial superoxide dismutase, expression increased with Cd treatment. Heme oxygenase 1 (HO-1) expression increased at low concentrations and decreased at higher concentrations of Cd.

Conclusion: The data suggest that Cd induces pyroptosis, oxidative stress and affects OXPHOS complex expression in RAW cells.