Bioactive Secondary Metabolites from Mangrove Endophytic Fungi
Mentor Information
Kristin Hererra (Chemistry)
Description
ESKAPE pathogens and Candida albicans are rapidly gaining antimicrobial resistance and can cause fatality in immunosuppressed patients. Endophytic fungi have been identified as critical sources for novel bioactive compounds, for example, novel terpenoids, alkaloids, quinones, xanthones, peptides, steroids, flavonoids, phenolic compounds have been identified. Many of these metabolites are novel and qualify as potential new pharmaceutical candidates for treatment of drug-resistant infections. EC10-33C-3B-HDAC has been epigenetically modified using histone-deacetylase, and then extracts were screened in ESKAPE bioassay showing strong broad-spectrum bioactivity. In this investigation, chemical extraction, separation, NMR and Liquid-Chromatography coupled to mass spectrometry will be employed to analyze potentially novel antimicrobial compounds. Fractions will be prioritized based on bioassay results towards both ESKAPE and Candida albicans. The MS-MS data will be compared to known substances in the GNPS (Global Natural Products Social Molecular Networking) to check for novelty.
Bioactive Secondary Metabolites from Mangrove Endophytic Fungi
ESKAPE pathogens and Candida albicans are rapidly gaining antimicrobial resistance and can cause fatality in immunosuppressed patients. Endophytic fungi have been identified as critical sources for novel bioactive compounds, for example, novel terpenoids, alkaloids, quinones, xanthones, peptides, steroids, flavonoids, phenolic compounds have been identified. Many of these metabolites are novel and qualify as potential new pharmaceutical candidates for treatment of drug-resistant infections. EC10-33C-3B-HDAC has been epigenetically modified using histone-deacetylase, and then extracts were screened in ESKAPE bioassay showing strong broad-spectrum bioactivity. In this investigation, chemical extraction, separation, NMR and Liquid-Chromatography coupled to mass spectrometry will be employed to analyze potentially novel antimicrobial compounds. Fractions will be prioritized based on bioassay results towards both ESKAPE and Candida albicans. The MS-MS data will be compared to known substances in the GNPS (Global Natural Products Social Molecular Networking) to check for novelty.
