A new subspecies of Amphipode is described, being the first representative of the Niphargus kochianus group in Bulgaria. The new subspecies resembles N. k. tamanini Ruffo, N. k. petrosani Dobr. et Manolache, N. k. labacensis Sket, N. k minor Sket, for the maxille I having one single hair on the internal lobe and a single combed spine on the external lobe. However, it differs neatly from all other subspecies by the length of the nails on the dactyl and by the peculiar form and the chetotaxia of the telson.