Identification and ß-lactam resistance in aquatic isolates of Enterobacter cloacae and their status in microbiota of Domica Cave in Slovak Karst (Slovakia)

Authors

Barbora GaalovaFollow
Alena DonauerovaFollow
Milan SemanFollow
Helena Bujdakova, Comenius University in BratislavaFollow

Abstract

Domica Cave is located in the Slovak Karst National Park in southern Slovakia. Heterotrophic cultivable psychrophilic and mesophilic microbiota were confirmed to be prevalent in this cave. Escherichia coli was the most abundant bacterium, followed by Enterobacter species and Serrratia species. Enterobacter cloacae isolates belong to the group of faecal contaminants (coliforms) of concern in water. Their status in cave ecosystem is questionable, but we observed E. cloacae in water samples all years round suggesting an autochthonous origin. We were concerned with possible contamination of the cave water with resistant E. cloacae from animal farms located 2-3 km from the cave. We tested 36 aquatic isolates of E. cloacae from Domica Cave to β-lactam resistance. The majority of tested isolates were resistant to more antibiotics (from 3 to 10), 5 isolates were resistant to more than 10 antibiotics, and 1 isolate was resistant to ampicillin. Resistance to the broad spectrum of β-lactams correlated with resistance mechanisms due to an expression of bla_TEM, bla_SHV and bla_CTX-M genes. The majority of isolates possessed a combination of tested resistance genes. We assume a direct impact of long-term human agricultural activities in the area of the Domica Cave to the conserved microbiota of karst system.

DOI

http://dx.doi.org/10.5038/1827-806X.43.1.7

Recommended Citation

Gaalova, Barbora; Alena Donauerova; Milan Seman; and Helena Bujdakova. 2014. Identification and ß-lactam resistance in aquatic isolates of Enterobacter cloacae and their status in microbiota of Domica Cave in Slovak Karst (Slovakia). International Journal of Speleology, 43: 69-77.
Available at: https://digitalcommons.usf.edu/ijs/vol43/iss1/7