The study of Mycetophilidae collected in Romanian caves during these past years enables us to add 24 species to those already known from the country, among which two are new for science: Exechia dumitrescui n. sp. and Rhymosia matilei n. sp. Sixteen species belonging to the genus Macrocera, Zelmira, Leia, Exechia, Rhymosia, Allodia, Mycetophila, Zygomyia and Deolpsis have never been mentioned before for the subterranean environment. Some of these species are extremely rare: Exechia januari Lundst., E. parallela Edw., Rhymosia tarnani Dzeid., Phronia kowarzi Dzicd., Mycetophila rudis Winn.