Document Type
Patent
Publication Date
November 2002
Patent Number
6482389
Abstract
A method and kit for determining candidates for immunotherapy, for monitoring the effect of immunotherapy and analysis of cell mediated immunity functionality in a patient who needs immunotherapy is provided. The method includes performing two intracutaneous skin tests and reading the skin test after twenty-four hours. One skin test is the administration of a mitogen such as phytohemagglutinin (PHA), concanavalin A (ConA), pokeweed antigen (PWA) and other mitogens as known in the art. The PHA skin test responses reflect the ability of the T-lymphocytes which are present to react to PHA and to release cytokines like IL-2 and induce a monocyte/macrophage infiltration leading to the DTH dermal reaction which is observed in the skin test characteristic of the afferent limb response of the immune system. The NCM (mitogen-stimulated natural cytokine mixture) skin test reflects the ability of preformed T-cell cytokines to induce the monocyte/macrophage accumulation characteristic of the efferent limb response.
Application Number
09/529,566
Recommended Citation
Hadden, John W., "Method to diagnose and monitor cellular immune deficiencies" (2002). USF Patents. 780.
https://digitalcommons.usf.edu/usf_patents/780
Assignees
University of South Florida
Filing Date
06/02/2000
Primary/U.S. Class
424/92
