The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.
Agrawal, Deepak Kumar; Pledger, Warren Jackson; Kluft, Jonathan A.; and Agrawal, Asha, "Electroporation buffer composition and method of use" (2013). USF Patents. 248.
H. Lee Moffitt Cancer Center & Research Institute, Inc. University of South Florida Proteacel, LLC