Aqueous Two-phase System, Water Structure, Liquid–liquid Phase Separation, Solvent Properties, Attenuated Total Reflection–fourier Transform Infrared Spectroscopy
Digital Object Identifier (DOI)
Analysis by attenuated total reflection–Fourier transform infrared spectroscopy shows that each coexisting phase in aqueous two-phase systems has a different arrangement of hydrogen bonds. Specific arrangements vary for systems formed by different solutes. The hydrogen bond arrangement is shown to correlate with differences in hydrophobic and electrostatic properties of the different phases of five specific systems, four formed by two polymers and one by a single polymer and salt. The results presented here suggest that the arrangement of hydrogen bonds may be an important factor in phase separation.
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Citation / Publisher Attribution
Biomolecules, v. 11, issue 12, art. 1787
Scholar Commons Citation
Madeira, Pedro P.; Titus, Amber R.; Ferreira, Luisa A.; Belgovskiy, Alexander I.; Mann, Elizabeth K.; Mann, Jay Adin Jr.; Meyer, William V.; Smart, Anthony E.; Uversky, Vladimir N.; and Zaslavsky, Boris, "Hydrogen Bond Arrangement Is Shown to Differ in Coexisting Phases of Aqueous Two-phase Systems" (2021). Molecular Medicine Faculty Publications. 999.