Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus
The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants.
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Citation / Publisher Attribution
Bioorganicheskaia Khimiia, v. 21, issue 7, p. 492-497
Scholar Commons Citation
Vysotskaia, Valentina S.; Nasibullin, U F.; Uversky, Vladimir N.; Vasilenko, K S.; Narizhneva, Natalya V.; and Garber, Marina B., "Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus" (1995). Molecular Medicine Faculty Publications. 630.