Transfection, Cytoplasmic Inclusions, Intrinsically Disordered Proteins, Small Interfering RNA, Cell Staining, Parkinson Disease, Vimentin, Prions
Digital Object Identifier (DOI)
Several human diseases including neurodegenerative disorders and cancer are associated with abnormal accumulation and aggregation of misfolded proteins. Proteins with high tendency to aggregate include the p53 gene product, TAU and alpha synuclein. The potential toxicity of aberrantly folded proteins is limited via their transport into intracellular sub-compartments, the aggresomes, where misfolded proteins are stored or cleared via autophagy. We have identified a region of the acetyltransferase p300 that is highly disordered and displays similarities with prion-like domains. We show that this region is encoded as an alternative spliced variant independently of the acetyltransferase domain, and provides an interaction interface for various misfolded proteins, promoting their aggregation. p300 enhances aggregation of TAU and of p53 and is a component of cellular aggregates in both tissue culture cells and in alpha-synuclein positive Lewy bodies of patients affected by Parkinson disease. Down-regulation of p300 impairs aggresome formation and enhances cytotoxicity induced by misfolded protein stress. These data unravel a novel activity of p300, offer new insights into the function of disordered domains and implicate p300 in pathological aggregation that occurs in neurodegeneration and cancer.
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Citation / Publisher Attribution
PLoS ONE, v. 7, issue 11, art. e48243
© 2012 Kirilyuk et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Scholar Commons Citation
Kirilyuk, Alexander; Shimoji, Mika; Catania, Jason; Sahu, Geetaram; Pattabiraman, Nagarajan; Giordano, Antonio; Albanese, Christopher; Mocchetti, Italo; Toretsky, Jeffrey A.; Uversky, Vladimir N.; and Avantaggiati, Maria Laura, "An Intrinsically Disordered Region of the Acetyltransferase P300 with Similarity to Prion-like Domains Plays a Role in Aggregation" (2012). Molecular Medicine Faculty Publications. 581.